Scientific Image Editing and Figure Creation (ONLINE)
courseFurtherInformation
This two-day online-course covers:
- Proper editing of scientific images for publications and good scientific image handling practice
- Understanding all aspects of digital images (e.g. file formats, metadata, bit depth, histogram, …)
- Proper merging of fluorescent channels and use of pseudo-colors and calibration bars
- Proper application of 2D projections from 3D data
- Optional: short glance on 3D reconstruction options in Fiji/ImageJ
- Scaling images to units (e.g. µm) via metadata and adding scale bars
- Uneven lighting correction methods for visualization
- Background correction methods
- Proper contrast adjustment respecting good scientific practice limits
- Avoiding data alterations when exporting images for publication figures
- Introduction to the vector graphic software Inkscape for efficient publication figure creation
- Creating example figures
All methods are learned hands-on with plenty of practical exercises to get confidence in applying them in your daily work.
During the course we will use a customized version of Fiji (ImageJ bundle) as well as Inkscape. Both are free, accessible for everybody and open source. Prior software knowledge is not required but might be of advantage.
The course has some focus on microscopic images. However, other image material such as Western Blots or Gel images will be integrated as well. Data visualization such as data plots are NOT part of this course!
To participate you will need:
- Computer or Laptop (best Windows or Linux)
- IMPORTANT: MacOS version 10.11 or newer (after 2018), older OS X versions do not support the newest Inkscape used in the workshop
- Stable internet connection
- Computer mouse and at least 1 big screen (you cannot properly follow with a laptop touch pad)
- Optimally:
- 2 monitors to watch the workinar on one and do the practical part on the other
- 2 monitors to watch the workinar on one and do the practical part on the other
- webcam and microphone
- Proficiency in handling your computer in general
- Possibility to install software on your computer (or administrator rights)
Software installation instructions and webinar access information will be communicated to registered participants prior to the workshop.
